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Signals below the background average (signal value <32) were considered non-expressing (Additional file 3).
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In Additional file 3 we present the top 100 candidates resulting from the SI analysis of the MAQC data, after filtering out all probesets expressed below background (average detection above background [DABG] p-value > 0.05).
miRNAs were eliminated from consideration if the average value of both β4 positive and β4 negative samples on a single microarray fell below the average background level detected on that particular microarray.
Genes having either the average Xn or Ym below the background level were excluded, as the true signal value is uncertain.
Therefore, features with signal-to-noise ratios (the background-subtracted average of feature pixels divided by the SD of local-background pixels) below 5 in both fluorescent channels were removed from further analysis.
Duplicate probes were removed, as were probes for unknown genes, and those for which the signal intensity in all 10 GeneChips was below 35 (the average background level).
The detection of responses (n = 8; 3× CMV and 5× FLU) was significantly better when the background was below the average background value (0.118 %) of the whole group (p = 0.003; Fig. 2).> -wrap-foot> The performance of each participant is depicted for the ICS proficiency panel phases 1 and 2 (and after central analysis of the phase 2 FCS files).
Bank lending is below the national average.
Still woefully below the national average.
Incomes are below the national average.
The average Central England Temperature was 7.6C, 0.9C below the 1981-2010 average but only 0.3C below the average since 1659.
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