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The methods used for handling concentrations below quantitation limits and missing covariate data must also be specified.
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Validation parameters of the method included accuracy, repeatability, precision, specificity, range, quantitation limit, and linearity.
The number of points below this quantitation limit is given and compared with the total number of observations.
Blood Pb levels began below the quantitation limit (usually ~ 1 μg/dL) and stayed very low in control animals throughout the course of the study.
GSDis were calculated using various methods for treating values below the analytical method detection and quantitation limits.
Statistical and visual analysis of the response obtained with the 0.01 pg/μL standard found that this ovine DNA concentration could not be distinguished from the background response of the NTC and was below the quantitation limit of the method.
The 0.1 pg/μL response was indistinguishable from the background response of the NTC and was determined to be below the quantitation limit.
The amounts of free light chain (L), free heavy chain (H) and of half antibody (HL) were below the quantitation limit.
The method was validated in terms of detection limits, quantitation limits (LOQs), linearity and precision.
The detection and quantitation limits of the guanine oxidation products are listed in Table 1.
Among the eight patients with HCV RNA titers below the quantitation limit, four remained positive by the chemiluminescence cRT-PCR, demonstrating the results in agreement with those using the nested RT-PCR.
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