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The proposal called "Votives in Suspension," for instance, realistically walks the viewer around an upper plaza, before stepping down, out of the sunlight, into a darkened memorial space below; "Passages of Light" and "Garden of Lights," by contrast, lift the viewer off the floor and let him zoom around the air as no ordinary pedestrian could ever hope to do, except perhaps in a dream.
All cells used in experiments were below passages 3. Passaged cells were plated at a concentration of 1×105 into 6 well plates containing DMEM with 10% FBS.
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Below are passages, describing the same events, from her novel "Thicker Than Water" and her memoir "The Kiss".
Fur tumor cell injections, cells below passage 10 were harvested by trypsinization, counted with a nucleocounter (Nucleocounter NC-200TM; ChemoMetec A/S, Allerod, Denmark), and prepared in solution of 106 cells/mL PBS.
Cells below passage 10 were designated "early passage cells".
Cells below passage 24 were used for experiments.
Subconfluent cultures (70 90% confluence) of lower passages (below passage 9) were utilized for the experiments described.
Normal human adult keratinocytes were purchased from TCS Cell Works, maintained in Epilife media supplemented with full supplement and antibiotics (Cascade Biologics Inc., USA) and used below passage ten.
It has been shown that early-passage (below passage 9) colonic primary fibroblasts maintain many of their in vivo characteristics, including expression of alpha-smooth muscle actin, collagen IV and laminin 1 [ 28].
All experiments were conducted using cells below passage 4. The cells were then reseeded in standard growth medium at 5 × 10 cells/cm onto substrates modified with different chemical functional groups and cultured to permit attachment and spreading.
All cells used were below passage 15 and cultured in Dulbecco's modified Eagle's medium containing 10% newborn calf serum, 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen, Breda, The Netherlands).
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