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Chemicals with more than 90% below detection limit values were excluded from analyses.
Congener sums assumed a value of 0 for below detection limit values.
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Several studies were excluded because there was a greater percentage of below-detection limit values, because of high detection limits or because the measurements were taken from studies much earlier than our time criteria.
Most of the missing (i.e., below the detection limit) values were in the 1T and 3T groups, indicating that they might be generally lower than in the controls.
There were three women at baseline, six women after the weight loss intervention at randomization, and 10 women at the 12-month weight loss maintenance phase that had estradiol concentrations below the detection limits (values <3 pg/ml).
When levels were below detection limit, numerical values of half the detection limit were used in the calculations.
If a sample concentration was below detection limit its value was set at the respective detection value.
IL-6 was dichotomised into below detection limit (<0.44 pg/mL) values and above detection limits (≥0.44), due to nearly 50% of values being below detection limit.
The graph is obtained by the pragmatic approach of replacing values below detection limit with the lowest robust value measured for each protein, and results are in close agreement with those obtained using other approaches (e.g. with rank observations, data not shown).
*P < 0.05 and **P < 0.01 vs. OVX (Mann-Whitney U - test) aNot detectable (values below detection limit).
In those cases we substituted the value of the detection limit for the data point; so, for cases where some samples were below detection limit, our reported averaged values are an upper bound to the true average value.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com