On a technical level, colonization with multiple strains might not always have been detected and colonization below detection level cannot be excluded.
In this cell line, STAT2 was below detection level in WB, and we did not detect an increase of MAP1LC3-II (Fig. 3; Fig. S2).
In contrast, Nck2 that was below detection level in CT36 and CT51, was distinctly detected in CT26, revealing increased expression of Nck2 in aggressive metastatic colon cancer cells.
The detection p-value was used to assign a call whether a measured transcript was detected at significant level as P (present), below detection level as A (absent) or in-between as M (marginal).
Simultaneously, individually differing species disappear below detection level while new species are temporally or constantly detectable during disease development [10], [11].
For example, rare groups that are still detected in sediments with high biomass may fall below detection level in sediments that have a lower biomass, resulting in erroneously low diversity indices [75].
In metastatic PDAC, the strongest ON expression was detected in the surrounding stroma, whereas it remained below detection level within the metastases.
Additionally, a stabilization of the hnRNP K-derived cleavage product was detected after 24-h puromycin treatment, when full-length hnRNP K was below detection level.
In the case of C the activity of N51A mutant was below detection level across the enzyme's concentration range.
Hydroxylamine and dithionite-HCl extractable C pools were, on average, small and often below detection level in temperate soils.
In Ung−/− cells the steady state level of uracil in genomic DNA is approximately 2000 per cell, while it was below detection level, and at least 10-fold lower, in wild-type cells (Nilsen et al., 2000).
