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From this, only interactions below a stringent cutoff [38] were used, resulting in a second set of 74,984 interactions.
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A stringent cutoff threshold for Q value of 5 × 10−2 was used to obtain high-confidence peaks.
Selecting the jointly top-ranked genes imposes a stringent cutoff for the identified genes, and reduces potential bias introduced due to local ancestry or population structure.
Even after applying a stringent cutoff, several miRNAs were retained as high scoring, statistically significant candidate regulators for various modules.
For example, with a stringent cutoff (R>0.4 and A<−0.4), we obtained 1863 NFRs with significant histone depletion.
To ensure only tightly correlated probes were clustered, we employed a stringent cutoff (correlation distance cutoff 0.2).
We use a P-value of 1e−5 or lower as a stringent cutoff.
The hit rate using a stringent cutoff of 2-fold inhibition (activity of <0.5) was 0.05%.
We chose a stringent cutoff to minimize the multiple testing problem.
We used a stringent cutoff for defining seropositivity, which probably underestimated the seroprevalence in our population.
Starting with a stringent cutoff, the joining was repeated with relaxed stringency in each successive step.
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