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Truncation below a fixed concentration threshold is known to introduce bias in half-life estimates for longitudinal data (Michalek et al. 1998) and is likely to have a similar effect in cross-sectional analyses.
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The enzyme immobilized nanoparticles were titrated with different concentrations of H2O2 and a fixed concentration of O-phenylenediamine (OPD).
Similarly, a fixed concentration of protein was incubated with increasing concentrations of ATP (ranging from 0 to 400 µM).
In this study, a fixed concentration of 26.3 µg/ml of EPI was selected for testing clinical isolates.
For the combination assays, IM was used at a fixed concentration of 0.6 µM with 0.4 or 0.8 µM Danusertib.
Purified DNA samples were diluted in 96-well microtiter source plates to a fixed concentration of 3 ng/ul.
A series of mixtures with a fixed concentration of aptamers (250 nM) and increasing concentrations of E2, GST, or E2-GST recombinant proteins was prepared.
In a second approach, a fixed concentration of labeled protein was incubated with increasing concentrations of BBMV from each strain (Fig. 2).
Level of inhibition was a function of PfClpY concentration at a fixed concentration of FI-peptide.
We also incubated different concentrations of E2-F tracer with a fixed concentration of ERα.
A fixed concentration of enzyme, nitrocefin, and increasing concentrations of inactivator were used in each assay.
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