Particularly, the RB family, to which Rbl2 belongs, regulates cell growth, differentiation and apoptosis, and is expressed in a cell cycle-dependent manner throughout spermatogenesis (Yan et al., 2001; Toppari et al., 2003).
If inside one of these windows we find a peak of our dataset we assume that the gene to which the transcript belongs is regulated by ERα binding to the selected peak.
Kim et al. have recently demonstrated that LDLR, the founding member of the LDLR gene family to which LRP1 belongs, negatively regulates apoE levels in the brain and promotes Aβ clearance in vivo [45].
The let-7 family, which let-7b and miR-202 belongs to, regulates oestrogen receptor alpha signalling in oestrogen receptor-mediated cellular malignant growth of breast cancer (Zhao et al, 2011), and is involved in self-renewal and tumourigenicity of breast cancer cells (Yu et al, 2007).
Genes differentially regulated belonged to almost all functional categories described by Journet et al. [ 22].
The genes that were differentially expressed in response to CC-3052 treatment belonged to pathways regulating cytokine-cytokine receptor interaction, Toll-like receptor signaling, complement and coagulation cascades, graft-versus-host disease, T cell receptor signaling, and apoptosis (Figure 3B).
Those members of this group, that have already been identified, belong to developmentally regulated genes.
We initially focused on temporal profiles of phosphorylation sites known to belong to pathways regulated by Akt and mTORC1 since, according to the data from our inhibitor screens, the PI3K-Akt/mTOR axis accounts for over half of the insulin-regulated phosphoproteome.
There were both up regulated and down regulated genes that belonged to this group and genes showing this characteristic behavior, AEN and CDKN1A (up in LDR and acute) and MYC and E2F5 (down in both LDR and acute), are shown in Fig. 3a and b, respectively.
Notably, 14% of the down regulated genes belonged to this category (Table S2).
