RSV was unable to activate PRDIII luciferase reporter activity in the cells transfected with empty vector or in those reconstituted with IKKγΔ (Figure 5D).
Unlike E2F1, E2F3 is unable to activate mTORC1, suggesting that growth activity could be restricted to individual E2F members.
As expected, MAVS was unable to activate NF-κB-driven luciferase reporter activity in IKKγ−/− MEFs, and mediated a 4-fold increase in IKKγ-WT transfectants (Figure 3B).
In contrast, tumor cells are unable to activate this protective response due to uncontrolled activation of growth pathways by oncogenic mutations.
In keeping with this observation, ERF.D.1 and ERF.D.2 are lacking the acidic activator domain and accordingly are unable to activate the synthetic GCC box promoter.
When amino acids are limiting, the EGO complex is unable to activate TORC1, leading to inhibition of growth and activation of protein degradation, stress-responses, and nitrogen scavenging pathways (Dann and Thomas 2006).
Trypsin, copper acetate and sodium dodecyl sulphate were unable to activate PPO, suggesting that the enzyme was in the active form.
Moreover, eNOS gene silencing decreased the mTOR activation by BCAAem in cells and BCAAem supplementation was unable to activate mTOR signaling in eNOS-/ mice [ 12].
When these TLR2-/ macrophages were evaluated with respect to the activation of MAP Kinase or NF-κB pathways, these ligands were unable to activate any pathways in these cells, indicating the TLR2-dependence of all these responses.
But when the latest iPhone — the 3G — arrived in stores around the world, systems became overwhelmed and many customers were unable to activate their new phones.
