This variant was characterized by an initial maintenance of damped oscillations and subsequent loss of rhythmicity after being transferred from light-dark (LD) cycles to constant darkness (DD).
These cells show no electrical activity (0 Hz) except when they are transferred from light to darkness; then an impulse is recorded, coincident with the transfer.
In treatment A (TA), smolts were transferred from continuous light to ambient short days (23 January 2001), whereas in treatment B, (TB) smolts were continued on 24-h light until natural day length approached the seasonal maximum (1 June 2001).
Meanwhile, after the leaves were transferred from intense light to darkness, the differences in the level of NPQ among the leaves treated with various concentrations of SHAM were abolished within one minute.
The remaining loci correspond to genes exhibiting a significant differential expression under a bud set experiment in which plants were transferred from constant light to short day conditions (Källman 2009).
When Synechococcus cells are transferred from the light to the dark, transcription of most of the genes on the genome is dramatically and rapidly suppressed (dark repression), while the transcription of a small subset (about 5%%) of genes is up-regulated (dark induction).
After the leaves were transferred from the intense light to darkness, the NPQ was quenched within one minute, and the difference in the level of NPQ between various concentrations of SHAM-treated leaves was abolished.
Fourteen-days old seedlings were transferred from 70 μmol m−2 s−1 light intensity and 8h day/ 16h night to 120 μmol m−2 s−1 continuous light.
Where replicates were transferred from darkness into the light there was a noticeable increase in stability but these differences were not significant.
For experiments assessing wheel-running activity in DD, mice were transferred from the breeding room (lights on 06:00 to 18 00 local time) to light-tight boxes and maintained in DD for at least 6 days with food provided ad libitum, and then were fasted for 48 hours starting at 18 00 local time.
Hamsters were transferred from SD to 13L 11D (lights on 0700 PST) at Week 8.
