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PC12 cells were thus cultured under differentiating conditions for either 12 h or 72 h before being stimulated with different values of hypergravity (50 g and 150 g).
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(a) Monocytes were stimulated with different concentrations of a recombinant protein (Tth) expressed in E. coli BL21 Star™ (DE3) or ClearColi® (CC) BL21 (DE3), or with different amounts of LPS (0.1 ng/ml ~1 EU/ml).
MDA-MB-231 (A,B) and PBMC (C,D) were stimulated with different concentrations of AA or DHA for 24 h (lines with black circle) or 48 h (lines with black square) (A D).
MDA-MB-231 (A) and 4T1 breast cells were stimulated with different concentrations of DHA for 24 h and cell death was assessed by labeling cells with annexin-V and propidium iodide followed by flow cytometry analysis.
Purified DCs (1 × 104 in 200 μL culture medium) were stimulated with different TLR ligands at the concentration mentioned above.
(A) The freshly isolated peritoneal macrophages (PEMs) were stimulated with different cytokines and LPS for 48 h.
The freshly isolated resting mouse peritoneal macrophages were stimulated with different cytokines in vitro, the expression of cytokines and chemokines were detected by microarray, real-time PCR, ELISA and multiple colors flow cytometry.
MK were stimulated with different amounts of PGN-Bio as well as with LPS for 48 h.
Freshly isolated T cells from WT and Olig1−/− MOG-immunized mice were stimulated with different concentrations of MOG.
Monocytes were stimulated with different TLR ligands with or without bovine IL-10 for 24 hours at 37°C and 5% CO2.
Secondly, when neurons were stimulated with different inter-stimulus intervals, peptide binding and unbinding rates were independent of the stimulus frequency.
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