Expression was stimulated about 6-fold 30 min after addition of the PGE2 (1 μ M) after which it rapidly returned to control levels.
The activities of mutants V174C, A404V, L441F, and M3 were stimulated about 1.5-, 2.9-, 2.1-foldd 2.1-fold by 0.2 0.5 M glucose, respectively, whereas the activity of mutant V174A was not stimulated by glucose.
However, VvSTS expression was enhanced much earlier in resistant V. riparia, where transcripts began to accumulate 12 h after inoculation and were stimulated about 20-fold at 2 days.
LC3-II formation was stimulated about threefold in incubations containing cytosol from starved WT MEFs and membranes from starved Atg5 KO MEFs, compared to incubations containing cytosol and membranes from non-starved MEFs.
In Hela cells transfected with pGL3BRCA1 plus the empty vector pCR3.1, the treatment with estrogen did not induce BRCA1 promoter activity, although it was stimulated about 2.0-fold upon cotransfection with pERα.
As shown before (Murayama and Uhlmann, 2014), cohesin's basal ATPase activity (sample 2) was stimulated about 3-fold by the presence of loader and DNA (sample 4), but only subtly increased by DNA alone (sample 3, Figure 1E).
LTB4 levels in OA osteoblasts were stimulated about twofold by 1,25-dihydroxyvitamin D3 (1,25(OH)2D3) plus transforming growth factor-β (TGF-β), a situation corresponding to their effect on FLAP mRNA levels.
thermoidea [ 31] were stimulated more than 2-fold by 0.05 0.2 M glucose; the β-glucosidases from Bacillus halodurans C-125 [ 9], uncultured bacterium [ 16], and Neotermes koshunensis [ 32] were stimulated about 1.6-, 1.3-, and 1.3-fold by 0.2 M glucose, respectively.
SlucS was stimulated about 2.5-fold by a cap, similar to the result reported by Timmer et al. [ 22] who compared the translation of uncapped and capped mRNA from a construct containing an α-globin ORF flanked by STNV-1 5′ and 3′ UTRs.
