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The three chromosomes are being sequenced by the Department of Energy's Joint Genome Institute (JGI) in Walnut Creek, California.
For instance, the number of genes in the N. crassa-orphan group is likely to be reduced upon the release of genomes from closely-related species, such as N. tetrasperma and N. discreta (currently being sequenced by the Joint Genomes Institute).
The genome of the cultivar Jemalong line A17 is currently being sequenced by the Medicago Genome Sequencing Consortium (MGSC) [ 6].
Using the NGS technologies, currently the genome of watermelon, which has an estimated size of 425 Mb [ 8], is being sequenced by the International Watermelon Genomics Initiative.
Clones sequenced by using transposon shotgun sequencing (TPS) include those that were finished by the TPS method after being sequenced by the PW method.
At a time when genomes are being sequenced by the hundreds, much attention has shifted from identifying genes and phenotypes to understanding the networks of interactions among genes.
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The enriched DNA was sequenced by the Ion Proton sequencer according to the manufacturer's protocols.
Purified PCR products were sequenced by the company BaseClear (Leiden, The Netherlands).
Several clones were sequenced by the dideoxynucleotide method with ABI-3730 automatic sequencer.
Isolated plasmids were sequenced by the cycle sequencing method using an ABI3700 96 capillary sequencer.
PCR products were sequenced by the dye terminator method and with an automatic sequencer.
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