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Although BCRP expression was enhanced in MCF-7 cells after being selected with either doxorubicin (MCF-7/AdrVp cells) or mitoxantrone (MCF-7/MX8 cells), a high level of efflux was noted with rhodamine 123 in MCF-7/AdrVp cells, whereas such efflux was not seen in MCF-7/MX8 cells [87].
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Transfection of the constructs was carried out as described before [32], [33] and parasites were selected with either 5 nM WR99210 or 2 µg/ml blasticidin (Invitrogen).
For epithelial cells, the epi cells were selected with either CD26-PE or CD104-PE.
With ME, the three gating functions were tested: independence function (indep), multinomial logit function (multinom) and the location function (loc) and the genes were selected with either t-test (1), RF (2), sPLS (3) for p* = 5. Figure 2 also displays the averaged error rate of some of the tested approaches where p* varies from 5 to 30.
Gene clusters were selected with either a minimal membership of 10 genes and a minimal correlation threshold of 0.7, or a minimum of 25 genes and a correlation of 0.6, respectively (for details see Additional file 4, Supplementary Methods Section 2).
They are being selected with an emphasis on equal male-female ratio, as per OIWW Global Standards.
Two different types of clones were selected with the two expression cassettes oriented either in same or the opposite direction (Fig. S1).
Genes with greater than twofold differences in gene expression were selected with a false discovery rate of either 0.05 or 0.01 [ 29].
If either Palmer or Leftwich is selected with the Cincinnati Bengals' No. 1 overall pick, difficult times are expected.
Clones containing pBS12086 were selected with thiostrepton.
Transfected cells were selected with puromycin for 4 days.
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