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MAb in ascite fluid was precipitated with 50% ammonium sulfate, centrifuged, and dialyzed against PBS before being purified using a protein G agarose column.
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Acetonitrile (MeCN) was purified using a known procedure [39, 40].
PCR products were purified using a PCR Clean-Up Kit (GMbiolab).
The protease fractions were purified using a 2D-Cleanup Kit.
The Pu stock solution was purified using a TEVA column.
The water was purified using a Millipore filtration system.
The product was purified using a PD-10 column and RCP was determined with SEC-HPLC.
DDI water was purified using a Barnstead E-pure (Model D4641) water system.
Finally, the DNA was purified using a QiaQuick PCR purification kit (Qiagen).
Plasmid DNA was purified using a LaboPass™ Plasmid Mini Purification Kit (Cosmo Bio Co., Ltd., Tokyo, Japan).
The mutant proteins were purified using a Ni-NTA column (Qiagen).
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