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After incubation for 2 h at room temperature while being protected from light, the suspensions were diluted and washed twice in PBS (pH 7.2) to remove all detectable free-FITC as determined by fluorescence measurement of the supernatants when compared to PBS alone.
The plates were again incubated for 10 min at 37°C while being protected from light.
Luminescent or fluorescent probes were added 15 minutes before measurements started, and samples were equilibrated while being protected from light.
Protein was labelled with a 3-fold excess of Atto 488 NHS ester dissolved in dry DMF for 1 h at room temperature while being protected from light.
OXi4503, kindly donated by OXiGENE (OXiGENE® Inc. South San Francisco, CA), was freshly prepared by dissolving in 0.9% sterile saline (NaCl) while being protected from light.
The cells (10/ml) were exposed to different experimental conditions and then submitted to an electrophoretic field (300 mA, 1 mV/cm) for 30 minutes at 4°C while being protected from light.
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The plasma samples were protected from light and stored at −80 °C before analysis.
FDP standard solution was protected from light due to its photosensitivity.
All products were protected from light and kept at room temperature.
The samples were protected from light and were incubated for 10 min in the dark, at 25 30 °C with RB (20 µM), as used for the PDI experiment.
For conjugation of CA72-4 mAb with CdSe/ZnS quantum dots with carboxyl group (Fig. 1a), all steps were protected from light to avoid fluorescent quenching of QDs.
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