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The pups were left under anaesthesia for a further 20 min before being processed, as described earlier.
To act as negative controls sections were also incubated with secondary antibody alone before being processed as described above.
To determine assay sensitivity, 10 BGC-823 cells were added to 10 mL of blood from healthy volunteersprior to being processed as described above for patients' samples.
After being processed as described in the "Materials and Methods" section, the high quality EST sequences were submitted into the dbEST (accession numbers: GH547439– GH547439).
Tissues were included in 4% agarose and serial coronal vibratome-cut sections (6 series, 40-μm-thick) were collected and stored in freezing solution (40% PBS, 30% ethylene glycol, 30% glycerol) before being processed as described below.
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The azo adduct formed was processed as described above.
The data have been processed as described earlier.
Organs were processed as described for the biodistribution study.
Tissues were processed as described above.
Cultured neurons were processed as described [12].
Embryos were processed as described [16].
More suggestions(15)
being processed as introduced
being developed as described
being constructed as described
being debated as described
being accepted as described
being loosened as described
being scored as described
being compared as described
being performed as described
being processed as written
being tested as described
being drawn as described
being read as described
being assayed as described
being distracted as described
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