Sentence examples for being probed with specific from inspiring English sources

Membranes were blocked in 1% BSA/5% non-fat milk in TTBS (10 mM Tris, pH 7.6, 150 mM NaCl, 0.1% Tween 20) for one hour at room temperature, before being probed with specific antibodies to phospho-IκBα (1 1000 dilution), IκBα (1 2,000) or β-actin (1 8,000).

Proteins of interest on the blots were probed with specific antibodies.

The sample was subjected to SDS-PAGE and transferred to a membrane that was probed with specific antibody.

After blocking with Tris-buffered saline containing 0.1% Tween 20 and 5% non-fat dry milk, membranes were probed with specific antibodies.

The membranes were probed with specific primary antibodies followed by anti-mouse or anti-rabbit secondary antibodies conjugated with harderadish peroxidase (Sigma).

To determine STAT6 activation in response to IL-9 in ASM cells, total cell protein was probed with specific anti phospho-tyrosine STAT6 and total STAT6.

Membranes were probed with specific antibodies at the dilutions listed above, followed by HRP conjugated goat anti-rabbit or anti-mouse IgG (Jackson ImmunoResearch Laboratories).

After blocking nonspecific reactivity, filters were probed with specific Abs diluted in TBS-T (20 mM Tris-HCl pH 8, 150 mM NaCl, and 0.05% Tween 20).

Proteins were resolved by SDS-PAGE and transferred to Immun-Blot PVDF membrane (BioRad Laboratories, Hercules, CA), and the blots were probed with specific antibodies as described earlier, followed by the appropriate species-specific secondary antibodies conjugated to HRP.

The membranes were probed with specific antibodies as indicated and then incubated with horseradish peroxidase-conjugated antibody against mouse and rabbit immunoglobulin (GE Healthcare), followed by detection with ECL Western blotting detection reagents (GE healthcare).

Blots were probed with specific antibodies HRP-conjugated anti-goat, anti-mouse and anti-rabbit antibodies (Santa Cruz Biotechnology) and the chemiluminescent substrate (Super Signaling West Pico Thermo Scientific, Pierce or ECL by Amersham, GE Healthcare) was used for detection.