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CHLA-9, CHLA-10, CHLA-25, CHLA-258 and SK-N-MC ES cells were transduced with lentiviral particles containing shRNA constructs encoding the ERBB4 shRNA and scrambled sequences and were selected with puromycin-containing media (1 μM; Sigma Aldrich), added every 2 days for 1 week before being plated for experiments.
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LNCaP and COS7 cells were maintained in RPMI 10% FBS, and 293T cells were maintained in DMEM 5% FBS, and all cells were plated for experiments in phenol red free RPMI+10% CSS.
Forty-eight hours after infection, cells were plated for subsequent experiments.
K562 cells (6 × 10) were plated for each experiment.
Neuroblastoma SH-SY5Y cells (1 × 10) were plated for each experiment.
The basal layer of a 7-day H53/H98 mixed SL-biofilm stained with SYTO 9 at the time cells were plated for the recombination experiment shown in Figure 5.
For TIRF microscopy experiments, cells were plated for 2 h on glass-bottom dishes (MatTek Corporation) precoated with 10 μg ml−1 of bovine plasma FN overnight at 4 °C.
Astrocytes were freshly plated for experiments and treated with 10 μM CCCP for 6 or 24 h.
Cells were then maintained without doxycycline for 2 3 passages before cells were plated and used for experiments.
After 5 days of culture, cells were plated and used for experiments the next day.
Cells were then counted and plated for experiments at the appropriate density in DMEM/F12 containing 2% FBS.
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