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After being picked with a micropipette, some single cell colonies were expanded in medium containing VEGF (Fig. 6A H) to evaluate the direct effect of this cytokine on the myogenic differentiation program of the leech myoendothelial cells.
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Operational taxonomic units (OTUs) were picked with a threshold of 97% pairwise identity.
Colonies were picked with a sterile loop and added to the drop.
Morphological different colony forming units (cfu) were picked with a sterile tip and streaked out onto fresh YM agar plates, repeatedly grown and picked for at least three times or until visible purity.
Citrate test was done were unique colonies were picked with a sterilized loop and streaked across the plates containing citrate media without breaking the agar and then incubated for 24 h.
For transplantation of CFU, 1 or 10 colonies, >70 µm in diameter were picked with a micromanipulator (CellTram Oil, Eppendorf).
The individual colonies were picked with a pipettor and subcultured in a 12-well plate with 1 ml culture medium for a week.
For colony PCR, a portion of a C. albicans colony was picked with a 10 µl pipette tip and mixed with 20 µl PCR reaction mixture; the initial step in the cycling program was altered to 5 min at 96°C.
Selected spots were picked with a 2 mm picking head.
Single plaques selected for purification were picked with a sterile toothpick.
Third-instar larvae of the appropriate genotype were picked with a cotton swab and placed in a clean vial.
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