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Four major parameters were investigated for their contributions to the response and separation of WM-5, with a total of 29 experiments being performed for each instrument, respectively.
Four parameters of UHPLC and six major parameters of ESI-MS were investigated for their contribution to analytes separation and response, leading to a total of 27 and 54 experiments being performed for each instrument, respectively.
A quality control procedure regarding image quality, protocol compliance, and completeness of study data is being performed for each vascular examination.
Subsequently, samples were centrifuged and supernatants were discarded, after which the resulting pellet was washed twice with 30 mL deionized water, with resuspension, centrifugation and supernatant removal being performed for each wash.
Each sample pool contained 200 300 nectaries, with two (MMN) or three (MLN and ILN) biological replicates being performed for each nectary tissue (see Table 1; each replicate was isolated from different plants).
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Six replicates were performed for each condition.
Parametrical optimization was performed for each step.
Five replicates were performed for each treatment.
Two serial passages were performed for each sample.
Four biological replicates were performed for each western blot assay.
Principal component analysis (PCA) was performed for each colony separately.
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