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Polyclonal sera have the advantage of typically being of higher affinity, but they are non-renewable resources.
It was noteworthy, however, that 0.1 units ml−1 brought about cell arrest and the start of cell death after 2 days (P<0.1) and a highly significant fall by day 4 (P≪0.01), indicating persistence of the enzyme as it gradually got to work with time (this enzyme being of higher affinity but lower capacity than arginase).
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Either model would predict that on a non-transgenic background, the affinity of the TCRs expressed by Itk null CD4+ T cells would tend to be of higher affinity so as to overcome the reduction in TCR signals in the absence of Itk.
Although the D2-like receptor agonist quinpirole is of higher affinity to DRD2, it can also activate DRD3 [ 49].
These ligand-binding protein interactions are of higher affinity than ligand-receptor interactions, preventing ligand-receptor binding unless disrupted by IGFBP proteases [ 26],[ 27].
This may be related to the affinity (Kd) of the gastrin receptors present on the xenograft lines as the Kds of the two xenografts inhibited by CR2093 were 4.6 x 10 -10 10 -1042J) and 1.2 x 10(-9) M (MKN45) respectively whereAR42Je Kd of C523 wandof higher affinity (2.2 x 1.2-10) M).
Assuming that both helical hook domains are active and, in accordance with the situation for SinR, SinI and SlrA, the hetero-oligomeric interactions are of higher affinity than the self-interactions, SlrR possesses the potential to form both "open" and "closed" complexes (Fig. 2).
Antibodies were of high affinity and capable of neutralising the bioactivity of hCG but were devoid of anti-peptide reactivity.
While the binding of the IFNAR2 receptor to interferon is of high affinity and well characterized, the binding to IFNAR1 is weak, transient, and poorly understood.
In the present study, a novel nano-platform (AuNC-cRGD-Apt) with dual targeting function was initially established by conjugating gold nanocluster (AuNC) with cyclic RGD (cRGD) that is specific to αvβ3integrins over-expressed on the surface of tumor tissues and aptamer AS1411 (Apt) that is of high affinity to nucleolin over-expressed in the cytoplasm and nucleus of tumor cells.
For two peptides, [Lys102]CFTR(102-116) and CFTR(1468-1480), antibody-peptide binding was of high affinity in that half-maximal binding occurred at peptide concentrations below 10 nM.
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