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Nuclei were stained with Prolong gold anti-fade reagent with DAPI (Molecular probes, Invitrogen) before being observed using a fluorescent microscope (Leica DMI3000).
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"Birds have never before been observed using a tool for more than one purpose," said Wimpenny.
It's the first time birds have ever been observed using a tool for more than one purpose.
The samples were observed using a microscope.
Fluorescence was observed using a confocal laser scanning microscope.
The sample morphology was observed using a SEM (LEO 1530VP).
Positive amplification was observed using a fluorescent detection reagent.
Flame behavior was observed using a high-magnification video system.
Selected minerals were observed using a petrographic microscope with cross-polarized light and a gypsum plate.
Cells were observed using a fluorescence microscope and an excitation wavelength of 350 nm.
The pathological changes were observed using a light microscope and photographs were obtained.
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