Since the mutant mice screened were maintained in several different genetic backgrounds, we first examined the impact of genetic background in our screen.
As such, highly inbred and presumably isogenic "clonal" lineages of this androdioecious species have long been maintained in several laboratories and used in a wide variety of experiments that require genetically uniform vertebrate specimens.
Confidentiality of data was maintained in several ways.
Currently, GRMD dogs are maintained in several colonies throughout the USA (including the University of Missouri and Texas A&M University, among others), and in France, Brazil and Australia.
The symbiovar tropici pSym shares an analogous history with symbiovar phaseoli pSym in the sense that a highly conserved plasmid has spread and is maintained in several different Rhizobium species [ 46].
As for the four MUs in the other ESU (R. Timmons, personal communication), Gila topminnows in Sharp Spring have gone extinct, apparently because of mosquitofish, but the Sharp Spring MU has been maintained in several populations at other sites and several captive populations.
We might attribute this discrepancy to differences in the method of DNA extraction or to evolutionary changes that occurred in the Oregon R strain, which has been maintained in various laboratories for several decades.
We show that viable colospheres, through their strong cell cell interactions, can be maintained in culture several weeks.
Eleutheria dichotoma medusae were collected in Southern France (Banyuls-sur-mer) and have been maintained in cultures for several years under constant laboratory conditions as described earlier [28].
There is no observable EC apoptosis during this process (Video S1) and stabilized EC networks can been maintained in culture for several weeks (data not shown).
