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The samples were then washed one more time before being imaged on a confocal laser microscope (Nikon A1R confocal, Nikon, Japan) using a 40x Nikon oil immersion objective lens.

(c) MDA-MB-231 cells transiently expressing the calcium probe (pGP-CMV-GCaMP6s) and adhering to FN were treated with an L-type calcium channel activator (BAY K8644; 1 μM) while being imaged on a TIRF microscope (63 × objective).

P53R172H PDAC cells transiently expressing the calcium probe (pGP-CMV-GCaMP6s) and adhering to FN were treated with an L-type calcium channel activator (BAY K8644; 1 μM) while being imaged on a TIRF microscope (1 picture every 10 s, 63x objective).

(d) MDA-MB-231 cells transiently expressing the calcium probe (pGP-CMV-GCaMP6s) and MYO10-mCherry were seeded on FN and treated with an L-type calcium channel activator (BAY K8644; 1 μM) while being imaged on a TIRF microscope (100 × objective; scale bar, 10 μm).

MDA-MB-231 cells transiently expressing the calcium probe (pGP-CMV-GCaMP6s) and adhering to FN were treated with an L-type calcium channel activator (BAY K8644; 1 μM) while being imaged on a TIRF microscope (1 picture every 10 s, 63x objective).

The resulting gels were removed, dried and exposed to Molecular Dynamics phosphor screens before being imaged on a Storm 720 scanner.

1011 vp of the IR800-labeled viruses were imaged on a Lumazone imaging system.

Gels were imaged on a Fujifilm gel imager using 1× Sybr gold stain.

For live-cell imaging, cells in chambers were imaged on a live cell station.

The gel was imaged on a Biorad Versadoc imager (Hercules, CA) using white light.

The membranes were imaged on a ChemiDoc XRS (Bio-Rad).