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After being homogenized with a rotor-stator homogenizer, tissue homogenates were transferred into a centrifuge tube and centrifuged at 12,000 rpm for 15 minutes at 4°C.
Briefly, following treatment cells were harvested and lysed using the Cytosol Extraction Buffer Mix with DTT and Protease Inhibitors, cells were incubated on ice for 10 min prior to being homogenized in a dounce homogenizer.
The water-in-oil emulsion that was formed was then placed in a glass syringe and added to 30 mL of ethylene oxide/propylene oxide block copolymer (Poloxamer-127; 2% [w/v] 30 mL); BASF, Mt. Olive, NJ) while the block copolymer was being homogenized by the probe homogenizer as described above for 15 minutes to form the water-in-oil-in-water emulsion.
The local has gone global, or in other words, anti-homogenization is itself now being homogenized: In Paris you find food trucks, in Gorky Park a snack kiosk called Williamsburg, there is a Brooklyn Diner in Dubai, and even the native beer, Brooklyn Brewery, is planning an outpost in Stockholm — how local is that?
Life on the planet is being homogenized by the expanding human population and the frequent and rapid movement of people and goods, which carry invasive organisms with them.
Soon he was exploring every corner of the Bronx, "blown away," he said, by "the diversity and all the ethnic enclaves that were still intact instead of being homogenized and watered down".
Similar(32)
Tissues were homogenized in tissue homogenizer.
The slices were homogenized using a Pro homogenizer (Oxford, CT).
Tissues were homogenized using a Kinematica homogenizer.
Tissues were homogenized using a tissue homogenizer.
The organs were homogenized by using a glass homogenizer.
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