Sentence examples for being frozen down from inspiring English sources

The resulting library was washed three times with PBS, before being frozen down in 15% glycerol.

On the same time we strongly suggest the further use of the same cell lines been transfected in this studies in Orthotopic Xenotransplantation Studies, as we best stable cells were frozen down already.

Aliquots were frozen down and thawed as needed for infections.

Cells were frozen down in multiple aliquots at passage 3 to 7, and stored in liquid nitrogen until use.

One of the resulting plates was frozen down and screening of clones was performed by Southern blots on the other plate, following digestion of isolated genomic DNA with BamHI or EcoRV and using "Rosa26-5'", a 502 bp probe, located upstream of the insert and amplified from the Rosa26 locus using the primers 5'-GATAGGAACTGGAAAACCAGAGGA-3' and 5'-GTAAGGGTCCAACAGAAAAGAGA-3' (Fig. 1A).

A sample was frozen down at each serial dilution.

When cultures reached confulence cells were frozen down in liquid nitrogen until further use.

Initially, cells were frozen down after every passage, however after passage 20 this was reduced to every five passages.

Every time a batch of cells is frozen down, it is recommended that one vial is resuscitated immediately to check viability.

Cells were washed in water and pellets of 1 2 × 10 cells were frozen down in liquid nitrogen and stored at 80°C until analysed.

After one week, the fibroblasts grew out from the NPC biopsies will be frozen down in liquid nitrogen for future use.