Sentence examples for being fixed overnight at from inspiring English sources

The cells were then incubated with CD1d/α-GalCer tetramer (ProImmune) for 20 min on ice followed by incubation with anti-B220 and anti-TCR-β antibodies for 15 min before being fixed overnight at 4°C in 4% formaldehyde (Sigma-Aldrich).

Batches of 40 100 larvae per group were fixed at 5 and 6 dpi for optical analyses.

Then the cells were washed again with PBS before being fixed with 70% ethanol at −2°C overnight.

Alpha and beta errors were fixed at 5 and 20%, respectively.

The tissues and embryonic stages were fixed over night in 4% paraformaldehyde in phosphate-buffered saline at 4°C.

The tissues and embryonic stages were fixed over night in 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS) at 4°C.

The Olympic's departure from Southampton had been fixed for yesterday at noon, but the fog that prevailed was denser than has been seen at Southampton for years, and the departure consequently was delayed until this morning, when it left in ideal weather.

For immunohistochemistry, embryos were fixed 2 4 h at 4 °C in 4% PF in PB.

Officials said the problem was fixed at 7.30pm on Saturday night but delays would continue throughout the weekend as airports worked through the backlog.

Cells were fixed at 0.5 h post 6.5 Gy irradiation and then incubated with the PE-conjugated antibody p-histone H2AX (S139) (Cell Signaling, Danvers, MA, USA) at 37 °C for 1 h.

Before sectioning, brains were fixed for at least 24 h at 4 °C in phosphate-buffered saline (PBS) containing 4% paraformaldehyde.