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Being extracts from plants and animals, they are biodegradable and unlikely to produce treated water with extreme pH [11].
Beyond being extracts and hence they are made up of a collection of phytochemicals, in contrast to vitamin C, these "teas" are used for health maintenance in various cultures.
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Following extraction, three main metal-ion fractions were obtained: i) buffer soluble (extracts 1 6); ii) acid soluble (extracts 7 9); iii) ash (non-soluble Zn and/or Cd).
For each run (96 extracts/run), extracts were tested using a duplex real-time PCR assay for EHV1 and ERV3 in the following reaction compositions; 10pmoles of each primer, 4pmoles of each probe (Table 1), 10 μL of SensiMix II Probe PCR Mix (Bioline, Australia) and 2 μL of extract in a 20 μL final reaction.
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