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Such sequences containing least Ns and not being extended on either end were defined as unigenes.
Such sequences without redundancy, containing the least amount of Ns and not being extended on either end, were defined as unigenes.
Paired-end reads were used again for gap filling of scaffolds to get sequences with least Ns and could not being extended on either end.
Then, these contigs in one transcript were assembled by the Trinity and gained the sequence not being extended on either end defined as unigenes.
After the steps mentioned above, the sequences obtained without redundancy, containing the least amount of Ns, and not being extended on either end were defined as unigenes.
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The consensus sequences without gaps and could not be extended on either end were termed as transcripts.
A hemispherical dome covers a central square, which is extended on either side so that the square forms the centre of a rectangle.
Sequences without Ns and which could not be extended on either end were defined as unigenes.
Finally, Trinity connected contigs and obtained sequences that could not be extended on either end.
Finally, Trinity connects the contigs, and gets sequences that cannot be extended on either end.
Sequences containing the least Ns and that could not be extended on either end were defined as unigenes.
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