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A parametric study was performed to illustrate the sensitivity of the eigenfrequency and damping on the foundation properties, the latter being expressed using the notion of dimensionless parameters (slenderness ratio and flexibility factor).
In this analysis, 9,841 genes were identified as being expressed using the Affymetrix technology (p ≤ 0.04) and 7,863 using MPSS.
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Each biosensor was expressed using the Tornado expression system in HEK293T cells, and total RNA was separated on a denaturing gel.
A recombinant H1 HA protein derived from the A/PR8 strain was expressed using the baculovirus expression system and purified using a His-Tag affinity column.
Myc-tagged human JNK3 was expressed using the pcDNA3-Myc6 mammalian expression plasmid.
HA (haemagglutinin -tagged haemagglutinin -taggedNK2 were expressed using thumanRα MKK4alian expression plasMKK7
Ig fusion proteins were expressed using the FreeStyle 293 expression system (Invitrogen) as described by the manufacturer.
Full-length KIF5B protein was expressed using the Bac-to-Bac expression system.
Protein was expressed using the Bac-to-Bac baculovirus expression system using a modification of a previously described protocol (17).
All transgenic constructs were expressed using the same backbone, thereby reducing the likelihood of differential expression in distinct cell types.
PNPO was expressed using the Thermo Scientific 1-Step Human Coupled IVT Kit according to manufacturer's instructions; this expression system uses a HeLa cell lysate.
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