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The vast majority of Wolbachia genes identified as differentially expressed across the D. melanogaster life cycle in the modENCODE time course showed a common pattern of being expressed at lower relative levels in embryos (75/80, 93.8%), with higher expression in either larval, pupal, and/or adult stages, and a transient decrease in expression at larval L3 (12 hr).
GFR α-2, the specific ligand-binding receptor for NTN was also found to be expressed in adult and ageing muscularis externa, with the GFR α-2(b) isoform being expressed at lower levels than the other two isoforms (Figures 3 and 4).
Similar to undifferentiated hESCs, most genes were expressed at relatively low levels with greater than 60% of the genes being expressed at lower than 50 tpm and 25% lower than 10 tpm.
In addition to being expressed at lower levels (Fig. 2), sex-biased genes also showed more variance in expression (Fig. 3), at least for genes mapped to autosomal locations.
Only one transgenic line, 13a, exhibited nondisjunction levels significantly different than the wild-type transgene, and this is likely a consequence of the mutant protein being expressed at lower levels than wild type (Table 1, Figure S2).
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Meanwhile, energy metabolism-related genes, which show aging-related decline in expression, are expressed at lower levels in females.
Second, we observed that PTEN expression was expressed at lower levels in BCBMs compared with other distant metastatic sites.
It was apparent that genes listed that showed high levels of expression inside epithelial cells were expressed at lower levels inside macrophages (Table 2).
In addition to expression in the eye, these genes are expressed at lower levels only in a few other tissues.
It seems clear that M protein and SIC are expressed at lower levels due to lowered expression of Mga.
Taken together, these results show that the target genes activated by AIRE are expressed at lower levels compared with the average expression level.
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