Also, all the data is exchanged using a secure connection, established by the Remote Attestation process.
In this regard, the data from the two ends of the protected zone can be exchanged using a high-speed (<1 ms) and reliable communication channel, where the time synchronization can be achieved by a timing signal obtained from a Global Positioning System transducer, whose delay tolerance is of the order of +/− 3 microsecond [21].
In this model, data are exchanged using a common format and protocol, usually XML and SOAP, respectively, between the host and any number of providers during the course of a pipeline execution.
For the initial crystallization studies, samples of protease that were stored in glycerol were exchanged using a Sephadex G25 column into 10 mM phosphate buffer, pH 7.45, containing 5 mM β-mercaptoethanol and then concentrated to 3 mg/mL.
The buffer was exchanged using a spin concentrator [10 kDa MWCO (molecular-mass cut-off); Sartorius] to buffer B [50 mM Tris/HCl (pH 7.8), 2 mM DTT and 10% (w/v) glycerol].
The information between the grids is exchanged using an algorithm presented in the paper.
Another 40percentt are "mediated", meaning non-identifying letters and photos are exchanged using an adoption agency or an attorney as a go-between.
E-mails can be exchanged using Postfix.
For centrifugal ultrafiltration, a 35 µl aliquot was buffer exchanged using a Vivaspin 0.5 ml device (10 kDa MWCO) at 12000 g and 4°C to a dilution factor (original buffer) of 7.5×105.
The cleaved protein was buffer exchanged using a PD10 column (GE Healthcare) into Ni-NTA-binding buffer (50 mm NaH2PO4 buffer, pH 8.0, 300 mM NaCl and 2.5 mM imidazole).
