Analysis of the primary miRNA transcript level using RT-qPCR showed an enrichment of these transcripts for the chimeric constructs, suggesting that the murine flanking regions used in this study result in structural changes that cannot be efficiently processed by Drosha/DGCR8 in the nuclear processing step.
Indeed, in a strain carrying a temperature-sensitive rsp5 allele, Def1 failed to be efficiently processed in response to 4-NQO at the restrictive temperature, whereas Def1 processing and accompanying Rpb1 degradation was observed in the WT control strain.
As the removal of terminal introns in spliced genes generally results in a significant inhibition of 3′end processing, it is unclear how naturally intronless pre-mRNAs can be efficiently processed at their 3′end.
The choice of patterns and symbols is important so that visual queries can be efficiently processed by the intended viewer.
However, when expressed as a fusion with the leader sequence from the ompA gene, hexahistidine-tagged PNGase F was efficiently processed and exported to the E. coli periplasm.
A recently introduced presintered cobalt-chromium (Co-Cr) alloy for metal ceramic restorations can be efficiently processed with computer-aided design/computer-aided manufacturing (CAD/CAM) techniques.
Furthermore, due to its metabolic activation, 35b was efficiently processed in liver cells compared to other cell types, including human cardiomyocytes.
At the same time, the DCs become loaded with antigenic proteins, that can be efficiently processed, without important effects on cell viability or altering the DC's phenotype and the cell's capacity to respond to danger signals.
User revocation can be efficiently processed and is suitable for e-health cloud application.
Consequently, SPARQL queries can be efficiently processed regardless of where the variables occur (subject, property, object) since one of the indexes will be applicable.
In this parallel algorithm, the system of linear tridiagonal equations, vector-vector additions and constant-vector multiplications are efficiently processed in parallel.
