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The third method of assessment was performed with PC12 cells being differentiated for five days in the presence of NGF and then exposed to viral vectors for three days in the continuous presence of NGF, followed by withdrawal of NGF and serum.
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Nsphs were differentiated for five days in differentiation medium [DMEM/F-12, N2, 1% penicillin/streptomycin and 0.5% FBS (Invitrogen)].
To investigate the effect of glutamate on oligodendrocyte differentiation, pre-committed neurospheres were differentiated for 3 days in the presence of glutamate receptor agonists.
Bone marrow cultures were differentiated for 1 week with additions of fresh differentiation medium at days 3 and 6.
In an initial experiment, H1 HESCs were differentiated for 30 days with RNA samples taken before and after differentiation.
The cells were differentiated for 6 days in order to reach an optimal cell density suitable for imaging.
Human neural progenitor cells (hNPC) were differentiated for 4 days in the presence of CeO2 or Sm-CeO2 (50 μg/mL).
The cells were differentiated for 4 days in the presence of CeO2 or Sm-CeO2 (50 μg/mL).
C17.2 cells were differentiated for and 7 days (10 days for GFAP) under continuous exposure to CeO2 (25 μg/mL), Sm-CeO2 (25 μg/mL) or NAC (1 mM).
C17.2 cells were differentiated for 6 days in the presence of CeO2 (25 μg/mL), Sm-CeO2 (25 μg/mL) or NAC (1 mM).
C17.2 cells were differentiated for 1 and 7 days under continuous exposure to CeO2 (25 μg/mL), Sm-CeO2 (25 μg/mL) or NAC (1 mM).
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