Sentence examples for being detached with from inspiring English sources

Following differentiation at 28 days of culture, 1 × 10 differentiated cells were harvested after being detached with 0.25% trypsin (Sigma-Aldrich®), washed with PBS, permeabilized in 4% of paraformaldehyde, and incubated for 18 h at 4°C with the monoclonal antibodies at the same dilutions described for immunostaining in 1% Triton X-100 and 0.5% bovine serum albumin (BSA).

After unsuccessful attempts to free the drill string, it was detached with explosives in 2808 m.

Cells on the micropatterned ITO electrodes could be detached with single-cell resolution.

Passage 4 MSCs were detached with 0.05% EDTA in 0.1% phosphate buffered saline (PBS) and rinsed with 0.1% PBS.

Although adhesions in the epidural space could be detached with epiduroscopy, adhesions recurred because of repeated epidural blocks following adhesiotomy.

The monolayer cells were detached with trypsin ethylene diamine tetra acetic acid (EDTA) to make single cell suspensions.

Intraoperatively after lensectomy and clearing of vitreous exudates, the temporal retina was seen to be detached with a large subretinal abscess with an area of overlying retinal necrosis.

The cells were maintained at 37 °C in a humidified incubator at 5%% CO2 and were detached with trypsin-EDTA solution (Thermo Fisher Scientific).

When the cells were 70% 80% confluent, they were detached with 0.05% trypsin EDTA (Gibco) and subcultured in 35 mm tissue culture dishes (Iwaki).

The capped molecules on the AgNPs surfaces were detached with the competitive absorption of chloride, and spontaneously triggered to form the controllable-density nanojunctions among the AgNPs aggregates.

Cells were detached with 0.25% trypsin/EDTA, washed in PBS.