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A number of 3 and 9-story, 3-D structural models being designed using the strength method, are considered.
An antenna allowing toroidal and poloidal steering over a wide range is being designed, using the ITER upper launcher steering mechanism.
The logical designs of the Online Collaborative Learning System or OCLS are being designed using the object-oriented models which are the use-case model and class diagram in order to show the concise processes of virtual "Think-Pair-Share" collaborative activities.
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The controller is designed using the linear optimal control theory.
Guide RNA (gRNA) sequences were designed using the Deskgen design platform (https://www.deskgen.com/guidebook/advanced.html).html
Primers used for mutation were designed using the QuikChange Primer Design Program (http://www.stratagene.com/sdmdesigner/default.aspx).aspx
All primers were designed using the Primer3Plus freeware (http://www.bioinformatics.nl/cgi-bin/primer3plus/primer3plus.cgi).
Primers for MreC mutants were designed using the NEBase ChangerTM tool (New England Biolabs, http://nebasechanger.neb.com).
Two pairs of primers were designed using the same strategy as described above for MreC mutations.
All qPCR primers were designed using the web-based Integrated DNA Technologies RealTime PCR Design Tool (http://idtdna.com/scitools/applications/realtimepcr).
Primers were designed using the PrimerQuest Tool (http://eu.idtdna.com/Primerquest/Home/Index) (Supplementary Table S5).
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