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After being cut using a vibratome, the slices were incubated at 33°C for 1 hr, and then held at room temperature (20 22°C) until being transferred to the recording chamber.
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Rectangular specimens (1 × 6 cm) were cut using a custom made cutting knife with razor blades.
For examination, sections were cut using a freezing microtome.
Nodular samples were cut using a trim saw.
After that, this plate is cut using a diamond wheel saw.
Leaf samples were cut using a sliding microtome, stained with Safranin-O and counterstained with Fast Green.
Transverse sections were cut using a cryostat (10 µm) (Leica, Wetzlar, Germany).
The standard geometry sizes of the bottom die were cut using a wire EDM machine.
Thin sections of 4 µm thicknesses are cut using a rotary microtome machine.
Sections from both sample types were cut using a diamond knife (Diatome) at a thickness between 200 and 300 nm.
10 µm-thick frozen tissue sections were cut, using a Leica CM3050 cryostat (Leica Microsystems, Milton Keynes, UK).
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