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The container was then moved into an oilbath; meanwhile, the temperature of the oilbath was kept at 80°C while being continuously stirred to achieve a viscose, clear, and honey-like gel.
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Direct sonication was performed for 45 min using amplitude of 90%% and 0.6 cycles; with the beaker containing cell suspension placed in ice/ice cold water and was continuously stirred to keep cool.
The solution was continuously stirred at fixed temperature for an assured time to attain the equilibrium.
Then, 2 mL of glycidyl methacrylate (Sigma Aldrich) was slowly added to the mixture, which was continuously stirred at 50 °C for two days under dry N2 gas.
In the next step, DCM was evaporated by elevating the emulsion temperature to 70 °C while the emulsion was continuously stirred at 200 rpm for 1 h.
The solution was continuously stirred at the same temperature for about 3 h and left on stirring over night at room temperature.
The pellet was washed three times for 15 min with 40 ml of ethanol and hexane (1 2, v/v) whilst being continuously stirred.
The mixture was continuously stirred at 200 rpm.
The obtained solution was continuously stirred at 70°C for 1 h.
The suspension was continuously stirred at room temperature under visible light irradiation.
Then, the conical flasks were continuously stirred at 25 °C for 24 h.
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