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The linkage map is being constructed using the F2 hybrids between the smooth (Lissotriton vulgaris vulgaris, Lvv) and Carpathian (Lissotriton montandoni, Lm) newts.
Later, the physical design of the prototype model is being constructed using the Web-based technologies which are the MySQL database, PHP and Apache web server.
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Phylogenetic trees were constructed using the MEGA5 package69.
All other PMFs were constructed using the transition rates method.
Index files were constructed using the bismark_genome_preparation command and the entire reference genome.
Tobacco BY-2 cells expressing CaMV 2 × pro35S::19E5scFv-GFP (H3K9ac-mintoboy-GFP) were constructed using the following procedures.
The untagged mmi1W112A strain was constructed using the approach outlined in Supplementary Fig. 4a.
All other Cl− PMFs were constructed using the distributed seeding approach to enhance sampling (see Methods).
Isopeptide-linked poly-SUMO1 chains were constructed using the Arabidopsis thaliana SUMO machinery components.
Orthogroups, or putative gene families, were constructed using the OrthoMCL method56.
For each sample, an RNA sequencing library was constructed using the Illumina mRNA-Seq Prep Kit.
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