However, in our hands, the ANTH domain of mammalian AP180 showed no significantly measurable binding to mammalian VAMPs 2, 3, or 8 by GST pull-downs or surface plasmon resonance (SPR), despite being correctly folded as indicated by circular dichroism (CD) (data not shown) and being competent to bind PtdIns(4,5 P2 similarly to CALMANTH by ITC and liposome-based SPR (data not shown).
Shi, F., Telesco, S. E., Liu, Y., Radhakrishnan, R. & Lemmon, M. A. ErbB3/HER3 intracellular domain is competent to bind ATP and catalyze autophosphorylation.
Conformational states that are competent to bind immune-stimulating FcγRs interconverted with Fc conformations distinct from those observed in FcγR complexes, which may represent a transient, nonbinding population.
Helices in Pik1(121-174) connected by an unstructured loop are colored magenta and the four EF-hands (EF1, EF2, EF3 and EF4) in Frq1 are highlighted in green, salmon, cyan, and yellow, respectively, but only EF2, EF3 and EF4 are competent to bind a calcium ion (orange sphere).
The openings are found in the active conformation, in which opsin is competent to bind and activate the G protein transducin.
Thus deletion of this region, as found in BDB mutants, yields a receptor which is competent to bind Wnt ligand but unable to produce a productive tyrosine phosphorylation signalling response.
While we found that there are substantial amounts of kermit2 in Xenopus embryos our GST pull downs and coimmunoprecipitations suggest that only a small fraction of kermit2 in the embryo is competent to bind to endogenous α5β1 integrin.
While all Skp1 isoforms were competent to bind Fbs1, glycosylated glycoforms bound preferentially.
Overall, our comparative analysis supports the prediction that the RGG box of Sho is competent to bind RNA.
Alternatively one kinetochore might be "silenced" so that only one kinetochore between the two sisters is competent to bind microtubules.
While it has been established that PrP is competent to bind nucleic acid, it is also known to bind many other ligands including polyanionic glycosaminoglycans ('GAGs').
