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The aorta was cut into circular strips, attached to a force transducer, and allowed to equilibrate for at least 1 hour before being challenged with a depolarizing saline solution replacing 51 mM NaCl with 51 mM KCl, to confirm viability and for normalization.
In brief mice were sensitized intraperitoneally on days 0 and 12 with 10 µg ovalbumin (Sigma, Poole, UK) and 2 mg aluminium hydroxide (Serva, Heidelberg, UK) or 2 mg aluminium hydroxide alone before being challenged with a nebulized aerosol of 5% ovalbumin in saline for 20 min on days 19 24 inclusive.
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The relentless emphasis over the last 40 years in public policy on economic growth and material gain is now being challenged with an increasing urgency.
A second group was challenged with a hand dynamometer, which measures grip strength.
And our attitude to foreign policy – and foreign military adventures – may be challenged with a broader historical understanding.
'Ex-gay' people who claim to have been cured will also be challenged with a sexuality lie detector test.
The theory seemed to some to have been proved when Crispin Blunt, the Reigate MP and former prisons minister, was challenged with a deselection attempt in September.
Finally, all aeration profiles were challenged with a 2-day storm through dynamic simulations.
After immunization, mice were challenged with a syngeneic melanoma expressing gp100, and tumor growth was analyzed.
After this procedure, fish were challenged with a 50% protein dilution (diets: PC50 and PF50).
The vaccinated chicks were challenged with a virulent Chinese field isolate.
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