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Antibody stained cells where washed using PBS and resuspended in 2% formaldehyde before being analyzed using a FACSCalibur flow cytometer (BD).
Crystalline phases were determined by X-ray powder diffraction (XRD), the XRD data being analyzed using a diffractometer with a Bragg Brentano geometry (Siemens D5000) equipped with a monochromator and based on cobalt Kα1 radiation (λ = 1.78897 Å).
Hybridization, processing, and scanning were performed by Filgen, Inc. (Nagoya, Japan), scan data images being analyzed using a software package (Microarray Data Analysis Tool, Filgen).
The cell suspensions were combined with 5 μl of annexin V-FITC and 5 μl of propidium iodide and incubated at room temperature for 5 min in the dark, before being analyzed using a BD FACSCanto II flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) using Mod Fit LT™ 3.3 software.
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The resulting solution was analyzed using a Shimadzu TOC-5000A analyzer.
Samples were analyzed using a 3100 Genetic analyzer (Applied Biosystems).
Plates were analyzed using a Molecular Devices ImageXpress High-Content Analysis SysteMolecular Devices ImageXpress High-Content
All samples were analyzed using a Leica SP5 confocal microscope.
Data were analyzed using a five-parameter logistic curve fit.
The data were analyzed using a PhosphorImager (Bio-Rad).
The samples were analyzed using a BD Accuri C6 flow cytometer.
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