Exact(3)
Following two PBS washes, cells were fixed in 0.1% formaldehyde overnight before being analysed using a FACScalibur flow cytometer (Beckton Dickinson).
The cells were then incubated with Alexa Fluor 488 donkey anti-goat secondary antibody (1/800 dilution) for 30 min at room temperature before being analysed using a flow cytometer.
The cotton swab was then centrifuged (Hettich Centrifuge EBA 20, Andreas Hettich Group, Ltd., Tuttlingen, Germany) at 2400 x g at room temperature for 2 minutes and the saliva extracted was stored at −20°C before being analysed using a commercially available cortisol ELISA kit (Cortisol ELISA, IBL International, Hamburg, Germany) validated for pig saliva [ 18].
Similar(57)
Blood count was analysed using a Sysmex analyser (XE 2100).
Attacks can be analysed using a mixture of digital forensics and traditional investigative methods.
The results were analysed using a statistical test.
Variables were analysed using a mixed model.
saplings were analysed using a multivariate method.
Differences were analysed using a linear mixed model.
Plasma samples were analysed using a validated analytical method.
PET data were analysed using a one-tissue compartment model.
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