At the start of the procedure mice were anaesthetised using a mixture of xylazine 12.5 mg.kg−1 (Bayer Pharma, Puteaux, France) and ketamine 125 mg.kg−1 (Parke Davis, Courbevoie, France).
Mice were anaesthetised using a mixture of xylazine 12.5 mg kg−1 (Bayer Pharma, Puteaux, France) and ketamine 125 mg kg−1 (Parke Davis, Courbevoie, France).
Each frog was anaesthetised using an aqueous solution of MS222 (Pharmaq Ltd ,UK) buffered with NaHCO3 to the pH of the water where the frog was collected (or at pH 7 if collected on land).
Mice were anaesthetised using an i.p. injection of 10 mg/kg xylazine (Bayer, Toronto, ON, Canada) and 200 mg/kg ketamine hydrochloride (Rogar, Montreal, QC, Canada).
The rats in each group were anaesthetised using an intraperitoneal (ip) injection of ketamine: xylazine (40 mg/kg: 5 mg/kg body weight; Serumwerk, Bernburg, Germany) injected with 37 MBq MIBI and imaged in two dynamic phases: a vascular phase at 1 sec/frame for 1 min and a subsequent parenchymal phase at 1 min/frame for 1 h.
Animals were anaesthetised using isoflurane which was delivered using an anaesthetic vaporiser.
Freshly caught field beetles were anaesthetised using CO2, and all the mites were removed and counted using a brush and tweezers.
Three to six day old male or female flies that had been socially housed (see [20]) were anaesthetised using CO2 and single flies transferred to a 5 mm ×65 mm polycarbonate tube containing food (see below).
Mice bearing LS174T (n=5) or SW1222 (n=5) xenografts were anaesthetised using isoflurane in air and secured in a cradle using dental paste to minimise motion (Landuyt et al, 2002; Burrell et al, 2011).
The patients were anaesthetised using propofol and remifentanil, and all patients had a left-sided, double-lumen endotracheal tube, an arterial line and a central venous line inserted.
