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The idea behind this calculation is that a small number of SNPs should show a true association with a trait of interest, while the other SNPs should follow the distribution under the null hypothesis of no SNP being associated [ 27].
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The rationale behind this calculation was that the edges (gene-pairs) found with high probabilities in resistant conditions but lower probabilities in parental conditions are more likely to be due to acquired resistance in cell lines.
For temperature, this calculation is relatively straightforward.
His goal in this calculation is simple.
Fortunately, this calculation is fairly straightforward.
This calculation is just basic subtraction.
Our trimming method approach allowed us to estimate the SD from the central part of the sample distribution, thus providing an alternative method; the statistical theory behind the calculation is described in the Appendix and it is relatively easy to apply to the data.
The math behind these calculations is not all that complicated.
A 3-dimensional PCA plot of the neuron development pathway, revealing the multivariate structure behind the outlier calculation, is shown in Additional file 2. We opted to determine the leading edge genes that distinguished each outlier group from the rest of samples.
This suggests that our calculation is valid.
Still, "The Limits to Growth" might be excused in spite of its lack of originality and scent of technical chicanery if those dismal assumptions behind the calculations were accurate.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com