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For behavioural trials, fish are removed from their home tank and placed into individual isolation chambers for at least 1.5 h before the trial.
While behavioural trials of working memory (WM) training have received much attention in recent years, a lesser explored parallel approach is functional neuroimaging.
Three generalist predators (crickets, soldier bugs and lynx spiders) that represent different feeding strategies were used in our individual-level repeated observation behavioural trials.
We suggest that future research investigate neural correlates of WM training in populations for which WM itself is impaired and/or behavioural trials of WM training have returned more promising results.
All crickets used in the behavioural trials were between 7 and 14 days old, post-eclosion.
Females to be used in behavioural trials were taken from the culture established in 2007 in order to minimize the inbreeding effects ensuing from prolonged laboratory rearing, whereas males were taken from the culture established in 2006.
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We repeated the behavioural trial over five consecutive days, using a new focal female each day.
The shoals were then placed back in the open-channel flume for a repeated behavioural trial post-infection.
For the behavioural trial by Cox 2008, the authors identified that the "walk and swim sessions were not separated according to the behavioural intervention.
Thus, startle-response deficits could potentially be used as an early quantitative behavioural test in preclinical trials involving both high and low-copy SOD1 G93A transgenic mice.
However, undertaking behavioural interventions trials in disadvantaged communities is often characterised by recruitment difficulties and robust design strategies for full trials would benefit from exploratory work as described in the MRC guidance for complex interventions (Craig et al., 2008).
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