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Behavioural parameters were recorded during weeks 4 and 5 of each experimental period, while cow performance was recorded during Week 5. Behavioural scans were taken at 30 min intervals during two non-consecutive 24-h periods in each recording week.
Behavioural scans were analysed on pen level (n = 80) and averaged over production phase (nursery phase: observations weeks 4, 5, and 8; finishing phase: observations weeks 11, 12, 16 and 21).
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A score of 1 was assigned to a male when it was displaying during at least one behavioural scan; otherwise a score of 0 was set.
The p-values indicating the deviations from exponentiality for behavioural elements scanning, walking, scanning-while-immobile, rear-up combined from 20 individual focal samples (10 males + 10 females) using Fisher's omnibus procedure are p = 0.01, p = 0.0001, p = 0.001, p = 0.001 respectively.
Parkinsonism and behavioural disturbances made scanning of the centrum semiovale and occipital lobe difficult, without negative effect on the temporal lobe examination.
From each participant, we obtained magnetic resonance imaging scans and behavioural data from a picture naming task that contrasted naming performance with living and non-living things as a way of manipulating perceptual and semantic confusability; living things are more similar to one another than non-living things, which have more distinctive features.
The behavioural patterns recorded using scan sampling were: (1) standing; (2) exploration: the horse walks slowly with its neck held horizontally or lowered, ready to stop and to sniff the ground or a wall.
Examination of the behavioural data collected during scanning showed that abnormal conditions were rated as being on average 2.05 (std = 0.1) and normal conditions were on average 2.15 (std = 0.08) on the aesthetic rating scale (1 = unappealing; 2 = neutral; 3 = appealing).
In all protocols, we used a combination of scan and behavioural sampling (Martin & Bateson 1986).
The scan and behavioural tests were carried out on the same day in 56% of cases and within a week of one another in 74% of cases.
MRI scanning and behavioural testing were conducted on average within a 3-month period (mean interval = 2.4 months, SD = 2.4 months).
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