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All fish were conditioned to accept agar gel pellet before the behavioural assays started.
The behavioural assays started after all fish were successfully conditioned to accept agar gel pellet.
Before the behavioural assays started, the fish were individually conditioned to accept agar gel pellet using the FT pellet.
The procedure of the behavioural assays in this study was adopted from that of Kasumyan and Morsi (1996) with modifications.
Tank cleaning was conducted and about 50%% of water in each aquarium was exchanged in the next day at least 4 h before the behavioural assays started.
The candidate chemicals are recommended for use in the subsequent part of the project, in which infochemical effects will be measured in established behavioural assays with invertebrates.
The fish were considered ready for the behavioural assays only when they consumed all the FT pellets in the continuous three days of training.
During the behavioural assays, the feeding responses of each fish to the given TS pellet (ingested or rejected) were observed and recorded.
Via chemical analyses and behavioural assays, we show that newly molted euhermaphrodite-phase shrimp contain a bouquet of odor compounds.
Headspace analysis of bacterial volatiles in combination with behavioural assays led to the identification of six compounds that elicited a behavioural effect in A. gambiae.
For details of behavioural assays, see (19).
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