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This trait ought to be retained by immortal hNSCs, if they are to provide a suitable, physiological model to mimic the behaviour of wild type hNSCs, while providing technical and practical advantages, such as long term and faster expandability in the absence of concomitant transformation.
This confirms that GCN4 translational regulation is essentially captured by the simplified Model 2, and that Factor X is not required to explain the translational behaviour of wild type GCN4 mRNA.
The aim is to provide a simple to use software tool focused on the straightforward application of several state-of-the-art flux analysis methods and the simulation of the behaviour of wild type or mutant strains.
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Given such wide diversity of observations, we have therefore aimed to characterise the full spectrum of behaviours of wild type and resistant virus that might be observed within a flexible model framework.
Towards this end, we compare the behaviour of wild-type cells to the response of two mutants, as shown in Figure 4.
The behaviour of wild-type and Oa1-deficient melanocytes also suggested that myosin Va was similarly recruited to melanosomes in both cell types.
As shown in Fig. 4A, we found a significant influence of rilmenidine treatment on the behaviour of wild-type mice in this test.
This fluorescent TEM-1 V216C mutant with wild-type activity can mimic the catalytic behaviour of wild-type TEM-1, thus allowing itself to serve as an in vitro drug screening tool.
Duplex and R-loop substrates containing PAM 5′-CCC were not bound by N153A appreciably better than substrates −PAM in EMSAs, contrasting with the binding behaviour of wild-type Cas8′.
Quantification of the dilute-like phenotype in pEGFP-MC-LT expressing cells revealed a comparable behaviour of wild-type and Oa1-deficient cell lines, either parental or transduced (Fig. 6B), supporting the notion that lack of OA1 function does not impair MT-based motility per se.
DOI: http://dx.doi.org/10.7554/eLife.03532.016 Because the electrophysiological behaviour of wild-type PapC usher is quite variable, and in attempt to quantify the propensity at spontaneous PD displacement, we have counted the number of 10-min long recordings (sweeps) that show 'large-open' behaviour, and we report the percent of such sweeps in various conditions.
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